Grayscale Investments LLC, which launched the Bitcoin Investment Trust in 2013, said NYSE Arca withdrew the application pending before the Securities and Exchange Commission to list the Trust shares.
The SEC denied two similar "Rule 19b-4" applications in March to bring to market a first-of-its-kind product tracking bitcoin, the digital currency.
Grayscale said it intends to continue its dialog with the SEC.
The withdrawal will not affect the Trust's quotation on the OTCQX, it said.
Bitcoin can be used to move money around the world quickly and with relative anonymity, without the need for a central authority such as a bank or government.
This article was provided by Reuters.